Identification of Zeatin and Zeatinriboside in Coconut Milk. Zeatin and zeatinriboside were identified from the milk of mature Cocos nucifera fruits. It would appear as if the two compounds are present in roughly equal proportions. Ethyl acetate extraction in a liquid-liquid extractor at an alkaline pH (8.0) proved to be a very efficient method of extracting zeatinriboside. Partitioning with water-saturated n-butanol proved to be the best way of extracting zeatin.
Author Information : Departments of Botany and Chemistry, University of Natal, Pietermaritzburg, South Africa
How to Cite STADEN, J. V. and DREWES, S. E. (1975), Identification of Zeatin and Zeatinriboside in Coconut Milk. Physiologia Plantarum, 34: 106–109. doi:0.1111/j.1399-3054.1975.tb03801.x
This article has been cited by:
1 Samira Chugh, Satyakam Guha, I. Usha Rao, Micropropagation of orchids: A review on the potential of different explants, Scientia Horticulturae, 2009, 122, 4, 507
2 Liya Ge, Jean Wan Hong Yong, Ngoh Khang Goh, Lian Sai Chia, Swee Ngin Tan, Eng Shi Ong, Identification of kinetin and kinetin riboside in coconut.., Journal of Chromatography B, 2005, 829, 1-2, 26
3 Jiangbo Wang, Denise M. Seliskar, John L. Gallagher, Tissue culture and plant regeneration of the salt marsh monocots Juncus roemerianus and Juncus gerardi, In Vitro Cellular & Developmental Biology - Plant, 2005, 41, 3, 274
4 Liya Ge, Jean Wan Hong Yong, Swee Ngin Tan, Xin Hao Yang, Eng Shi Ong, Analysis of some cytokinins in coconut (Cocos nucifera L.) water by micellar electrokinetic capillary chromatography after solid-phase extraction, Journal of Chromatography A, 2004, 1048, 1, 119
5 Xin Y. Li, Feng H. Huang, Induction of somatic embryogenesis in loblolly pine (Pinus taeda L.), In Vitro Cellular & Developmental Biology - Plant, 1996, 32, 3, 129
6 H. Kobayashi, N. Morisaki, Y. Tago, Y. Hashimoto, S. Iwasaki, E. Kawachi, R. Nagata, K. Shudo, Identification of a major cytokinin in coconut milk, Experientia, 1995, 51, 11, 1081
7 M.B. Jackson, Advances in Botanical Research Volume 19, 1993,
8 Gilberto B. Kerbauy, In vitro Conversion of Cattleya Root Tip Cells into Protocorm-like Bodies, Journal of Plant Physiology, 1991, 138, 2, 248
9 Curriculum Vitae Et Studiorum, Giornale botanico italiano, 1988, 122, sup002, 7
10 Roberto Lorenzi, Paolo Vernieri, Nello Ceccarelli, Cytokinins in Endosperm of Sechium edule Sw. Seeds, Journal of Plant Physiology, 1988, 133, 3, 310
11 S. U. REMISON, Effect of gibberellic acid, kinetin and naphthylacetic acid on the growth of coconut seedlings, Annals of Applied Biology, 1984, 105, 2
12 Allan E. Stafford, Judith A. Kunhle, Joseph Corse, Earl Hautala, Isolation and purification of plant hormones by XAD-2 column chromatography, Journal of Chromatography A, 1984, 294, 485
13 Roger J. Gautheret, Plant tissue culture: A history, The Botanical Magazine Tokyo, 1983, 96, 4, 393
14 J. Staden, Seeds and cytokinins, Physiologia Plantarum, 1983, 58, 3
15 D.S. Letham, A 6-oxypurine with growth promoting activity, Plant Science Letters, 1982, 26, 2-3, 241
16 L. Dix, J. Staden, Auxin and gibberellin-like substances in coconut milk and malt extract, Plant Cell, Tissue and Organ Culture, 1981, 1, 1, 239
17 Maurice F. Barnes, Cheng Lu Tien, John S. Gray, Biosynthesis of cytokinins by potato cell cultures, Phytochemistry, 1980, 19, 3, 409
18 J. STADEN, J. E. DAVEY, The synthesis, transport and metabolism of endogenous cytokinins, Plant, Cell & Environment, 1979, 2, 2
19 J. Staden, The nature of a cytokinin in potato tubers, Potato Research, 1976, 19, 3, 249
20 J. Van Staden, Cytokinins from larvae in Erythrina latissima galls, Plant Science Letters, 1975, 5, 4, 227
Author Information : Departments of Botany and Chemistry, University of Natal, Pietermaritzburg, South Africa
How to Cite STADEN, J. V. and DREWES, S. E. (1975), Identification of Zeatin and Zeatinriboside in Coconut Milk. Physiologia Plantarum, 34: 106–109. doi:0.1111/j.1399-3054.1975.tb03801.x
This article has been cited by:
1 Samira Chugh, Satyakam Guha, I. Usha Rao, Micropropagation of orchids: A review on the potential of different explants, Scientia Horticulturae, 2009, 122, 4, 507
2 Liya Ge, Jean Wan Hong Yong, Ngoh Khang Goh, Lian Sai Chia, Swee Ngin Tan, Eng Shi Ong, Identification of kinetin and kinetin riboside in coconut.., Journal of Chromatography B, 2005, 829, 1-2, 26
3 Jiangbo Wang, Denise M. Seliskar, John L. Gallagher, Tissue culture and plant regeneration of the salt marsh monocots Juncus roemerianus and Juncus gerardi, In Vitro Cellular & Developmental Biology - Plant, 2005, 41, 3, 274
4 Liya Ge, Jean Wan Hong Yong, Swee Ngin Tan, Xin Hao Yang, Eng Shi Ong, Analysis of some cytokinins in coconut (Cocos nucifera L.) water by micellar electrokinetic capillary chromatography after solid-phase extraction, Journal of Chromatography A, 2004, 1048, 1, 119
5 Xin Y. Li, Feng H. Huang, Induction of somatic embryogenesis in loblolly pine (Pinus taeda L.), In Vitro Cellular & Developmental Biology - Plant, 1996, 32, 3, 129
6 H. Kobayashi, N. Morisaki, Y. Tago, Y. Hashimoto, S. Iwasaki, E. Kawachi, R. Nagata, K. Shudo, Identification of a major cytokinin in coconut milk, Experientia, 1995, 51, 11, 1081
7 M.B. Jackson, Advances in Botanical Research Volume 19, 1993,
8 Gilberto B. Kerbauy, In vitro Conversion of Cattleya Root Tip Cells into Protocorm-like Bodies, Journal of Plant Physiology, 1991, 138, 2, 248
9 Curriculum Vitae Et Studiorum, Giornale botanico italiano, 1988, 122, sup002, 7
10 Roberto Lorenzi, Paolo Vernieri, Nello Ceccarelli, Cytokinins in Endosperm of Sechium edule Sw. Seeds, Journal of Plant Physiology, 1988, 133, 3, 310
11 S. U. REMISON, Effect of gibberellic acid, kinetin and naphthylacetic acid on the growth of coconut seedlings, Annals of Applied Biology, 1984, 105, 2
12 Allan E. Stafford, Judith A. Kunhle, Joseph Corse, Earl Hautala, Isolation and purification of plant hormones by XAD-2 column chromatography, Journal of Chromatography A, 1984, 294, 485
13 Roger J. Gautheret, Plant tissue culture: A history, The Botanical Magazine Tokyo, 1983, 96, 4, 393
14 J. Staden, Seeds and cytokinins, Physiologia Plantarum, 1983, 58, 3
15 D.S. Letham, A 6-oxypurine with growth promoting activity, Plant Science Letters, 1982, 26, 2-3, 241
16 L. Dix, J. Staden, Auxin and gibberellin-like substances in coconut milk and malt extract, Plant Cell, Tissue and Organ Culture, 1981, 1, 1, 239
17 Maurice F. Barnes, Cheng Lu Tien, John S. Gray, Biosynthesis of cytokinins by potato cell cultures, Phytochemistry, 1980, 19, 3, 409
18 J. STADEN, J. E. DAVEY, The synthesis, transport and metabolism of endogenous cytokinins, Plant, Cell & Environment, 1979, 2, 2
19 J. Staden, The nature of a cytokinin in potato tubers, Potato Research, 1976, 19, 3, 249
20 J. Van Staden, Cytokinins from larvae in Erythrina latissima galls, Plant Science Letters, 1975, 5, 4, 227
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